Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
- (1)
- (1)
- (3)
- (1)
- (115)
- (1)
- (2)
- (1)
- (2)
- (2)
- (72)
- (2)
- (1)
- (1)
- (1)
- (1)
- (10)
- (3)
- (2)
- (1)
- (10)
- (6)
- (2)
- (4)
- (2)
- (10)
- (1)
- (5)
- (5)
- (113)
- (107)
- (1)
- (1)
- (1)
- (1)
- (1)
- (11)
- (2)
- (7)
- (1)
- (1)
- (1)
- (2)
- (6)
- (8)
- (23)
- (1)
- (12)
- (21)
- (1)
- (4)
- (1)
- (12)
- (1)
- (2)
- (1)
- (54)
- (6)
- (5)
- (34)
- (129)
- (2)
- (1)
- (27)
- (2)
- (1)
- (6)
- (1)
- (1)
- (2)
- (19)
- (2)
- (1)
- (26)
- (1)
- (1)
- (6)
- (7)
- (3)
- (8)
- (1)
- (89)
- (155)
- (8)
- (6)
- (10)
- (1)
- (1)
- (4)
- (1)
- (1)
- (1)
- (1)
- (1)
- (2)
- (9)
- (22)
- (155)
- (6)
- (3)
- (1)
- (4)
- (2)
- (3)
- (7)
- (5)
- (3)
- (9)
- (4)
- (616)
- (5)
- (4)
- (4)
- (129)
- (23)
- (32)
- (49)
- (6)
- (4)
- (2)
- (5)
- (125)
- (2)
- (14)
- (10)
- (3)
- (14)
- (2)
- (49)
- (1)
- (1)
- (2)
- (32)
- (13)
- (5)
- (26)
- (2)
- (4)
- (9)
- (9)
- (23)
- (45)
- (5)
- (56)
- (5)
- (13)
- (1)
- (3)
- (2)
- (3)
- (1)
- (9)
- (1)
- (2)
- (3)
- (93)
- (1)
- (1)
- (3)
- (4)
- (4)
- (3)
- (3)
- (3)
- (10)
- (3)
- (31)
- (4)
- (3)
- (9)
- (6)
- (2)
- (45)
- (1)
- (3)
- (1)
- (4)
- (1)
- (1)
- (2)
- (1)
- (1)
- (3)
- (5)
- (1)
- (2)
- (4)
- (3)
- (5)
- (1)
- (5)
- (10)
- (10)
- (6)
- (1)
- (4)
- (2)
- (5)
- (3)
- (2)
- (3)
- (5)
- (10)
- (1)
- (2)
- (1)
- (2)
- (18)
- (7)
- (1)
- (2)
- (3)
- (1)
- (2)
- (2)
- (1)
- (1)
- (1)
- (2)
- (2)
- (5)
- (7)
- (1)
- (2)
- (10)
- (4)
- (2)
- (1)
- (1)
- (7)
- (3)
- (3)
- (1)
- (2)
- (1)
- (1)
- (3)
- (2)
- (2)
- (1)
- (3)
- (2)
- (1)
- (13)
- (4)
- (1)
- (4)
- (3)
- (2)
- (2)
- (2)
- (1)
- (4)
- (2)
- (2)
- (6)
- (1)
- (1)
- (3)
- (10)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (14)
- (1)
- (2)
- (38)
- (2)
- (1)
- (1)
- (2)
- (1)
- (2)
- (2)
- (11)
- (8)
- (8)
- (2)
- (1)
- (2)
- (3)
- (1)
- (3)
- (2)
- (3)
- (1)
- (32)
- (1)
- (5)
- (8)
- (3)
- (2)
- (14)
- (5)
- (3)
- (3)
- (1)
- (5)
- (1)
- (22)
- (3)
- (1)
- (66)
- (11)
- (1)
- (1)
- (43)
- (1)
- (13)
- (2)
- (1)
- (1)
- (2)
- (1)
- (2)
- (1)
- (11)
- (1)
- (39)
- (2)
- (1)
- (1)
- (10)
- (1)
- (1)
- (14)
- (1)
- (2)
- (1)
- (1)
- (21)
- (1)
- (5)
- (3)
- (1)
- (17)
- (3)
- (1)
- (4)
- (1)
- (3)
- (1)
- (4)
- (1)
- (6)
- (22)
- (3)
- (3)
- (80)
- (2)
- (49)
- (1)
- (3)
- (8)
- (6)
- (20)
- (40)
- (3)
- (7)
- (12)
- (138)
- (12)
- (5)
- (30)
- (6)
- (34)
- (8)
- (37)
- (6)
- (1)
- (7)
- (7)
- (17)
Filtered Search Results
Invitrogen™ Novex™ Tris-Glycine Native Running Buffer (10X)
Use with NuPAGE Novex Tris-Acetate gels
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.4
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00132476 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Invitrogen™ Novex™ Tris-Glycine Native Sample Buffer (2X)
Ideal for native running conditions with NuPAGE Tris-Acetate gels
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, ultrapure, 99.9%
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | TRIS |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 8.5, 0.2 micron filtered
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals Glycine, 0.2M buffer soln., pH 2.5
CAS: 56-40-6 Molecular Formula: C2H5NO2 Molecular Weight (g/mol): 75.07 MDL Number: MFCD00008131 InChI Key: DHMQDGOQFOQNFH-UHFFFAOYSA-N IUPAC Name: 2-aminoacetic acid SMILES: NCC(O)=O
| CAS | 56-40-6 |
|---|---|
| Molecular Weight (g/mol) | 75.07 |
| MDL Number | MFCD00008131 |
| SMILES | NCC(O)=O |
| IUPAC Name | 2-aminoacetic acid |
| InChI Key | DHMQDGOQFOQNFH-UHFFFAOYSA-N |
| Molecular Formula | C2H5NO2 |
Invitrogen™ Tris (1 M), pH 7.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Formulation | 1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-,Hydrochloride |
|---|---|
| Physical Form | Liquid |
| pH | 7 |
| Grade | Molecular Biology |
| Recommended Storage | Store at room temperature. |
| Concentration | 1 M |
| Product Line | Ambion |
Invitrogen™ TE, pH 7.0, RNase-free
Invitrogen TE buffer is a commonly recommended storage solution for RNA samples. 10 mM Tris-HCl, 1 mM EDTA, pH 7.0 is in nuclease-free, ultrapure water.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Packaging | Bottle |
|---|---|
| Physical Form | Liquid |
| pH | 7.0 |
| Recommended Storage | Store at room temperature. |
| Product Line | Ambion |
Thermo Scientific Chemicals Krebs-Ringer Solution, HEPES-buffered
Standard physiological buffer that is suitable for various calcium imaging experiments. It is also used as a preserving agent.
| Solubility Information | Not miscible or difficult to mix in water. |
|---|---|
| Packaging | Plastic bottle |
| Physical Form | Liquid |
| Chemical Name or Material | Krebs-Ringer Solution |
| TSCA | Yes |
| Recommended Storage | Ambient temperatures |
Invitrogen™ Novex™ Tricine SDS Sample Buffer (2X)
Use with Novex Tricine Gels
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Thermo Scientific™ Pierce™ IgG Elution Buffer, pH 2.0
Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.
Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 7.5
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
| PubChem CID | 23831 |
|---|---|
| CAS | 7365-45-9 |
| Molecular Weight (g/mol) | 238.30 |
| ChEBI | CHEBI:42334 |
| MDL Number | MFCD00006158 |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O4S |
Gibco™ PBS, pH 7.2, No Sodium Pyruvate
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Gibco PBS, pH 7.2, is a balanced salt solution widely used in various cell culture applications to maintain a stable pH and osmotic balance, making it ideal as a wash, transport, and dilution buffer.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Includes | No Sodium Pyruvate |
|---|---|
| Color | White |
| Physical Form | Liquid |
| pH | 7.2 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Concentration | 1X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 280 to 320 mOsm/kg |
| Sterility | Sterile |
| Solution Type | pH Buffer, Storage Solution and Cleaning Solution |
| Product Line | Gibco |
Tris(hydroxymethyl)aminomethane (Cryst./Certified ACS), Fisher Chemical™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-(hydroxymethyl)-1, 3-propanediol,Trimethylol Aminomethane,Tris Buffer,Tris,Trizma™,THAM,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | 2-Amino-2-(hydroxymethyl)-1, 3-propanediol,Trimethylol Aminomethane,Tris Buffer,Tris,Trizma™,THAM,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Tris Hydrochloride, 1M Solution (pH 7.0/Mol. Biol.), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | Tris-HCl |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |